Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/36230
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dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorDong, YZ-
dc.creatorZhang, LJ-
dc.creatorWu, ZM-
dc.creatorGao, L-
dc.creatorYao, YS-
dc.creatorTan, NZ-
dc.creatorWu, JY-
dc.creatorNi, LQ-
dc.creatorZhu, JS-
dc.date.accessioned2016-04-15T08:36:51Z-
dc.date.available2016-04-15T08:36:51Z-
dc.identifier.urihttp://hdl.handle.net/10397/36230-
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rights© 2014 Dong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rightsThe following publication: Dong Y-Z, Zhang L-J, Wu Z-M, Gao L, Yao Y-S, Tan N-Z, et al. (2014) Altered Proteomic Polymorphisms in the Caterpillar Body and Stroma of Natural Cordyceps sinensis during Maturation. PLoS ONE 9(10): e109083 is available at https://doi.org/10.1371/journal.pone.0109083en_US
dc.titleAltered proteomic polymorphisms in the caterpillar body and stroma of natural Cordyceps sinensis during maturationen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume9en_US
dc.identifier.issue10en_US
dc.identifier.doi10.1371/journal.pone.0109083en_US
dcterms.abstractObjective: To examine the maturational changes in proteomic polymorphisms resulting from differential expression by multiple intrinsic fungi in the caterpillar body and stroma of natural Cordyceps sinensis (Cs), an integrated micro-ecosystem.-
dcterms.abstractMethods: The surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) biochip technique was used to profile the altered protein compositions in the caterpillar body and stroma of Cs during its maturation. The MS chromatograms were analyzed using density-weighted algorithms to examine the similarities and cluster relationships among the proteomic polymorphisms of the Cs compartments and the mycelial products Hirsutella sinensis (Hs) and Paecilomyces hepiali (Ph).-
dcterms.abstractResults: SELDI-TOF MS chromatograms displayed dynamic proteomic polymorphism alterations among samples from the different Cs compartments during maturation. More than 1,900 protein bands were analyzed using density-weighted ZUNIX similarity equations and clustering methods, revealing integral polymorphism similarities of 57.4% between the premature and mature stromata and 42.8% between the premature and mature caterpillar bodies. The across-compartment similarity was low, ranging from 10.0% to 18.4%. Consequently, each Cs compartment (i.e., the stroma and caterpillar body) formed a clustering clade, and the 2 clades formed a Cs cluster. The polymorphic similarities ranged from 0.51% to 1.04% between Hs and the Cs compartments and were 2.8- to 4.8-fold higher (1.92%-4.34%) between Ph and the Cs compartments. The Hs and Ph mycelial samples formed isolated clades outside of the Cs cluster.-
dcterms.abstractConclusion: Proteomic polymorphisms in the caterpillar body and stroma of Cs change dynamically during maturation. The proteomic polymorphisms in Hs and Ph differ from those in Cs, suggesting the presence of multiple Cs-associated fungi and multiple Ophiocordyceps sinensis genotypes with altered differential protein expression in the Cs compartments during maturation. In conjunction with prior mycological and molecular observations, the findings from this proteomic study support the integrated micro-ecosystem hypothesis for natural Cs.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationPLoS one, 2014, v. 9, no. 10, e109083-
dcterms.isPartOfPLoS one-
dcterms.issued2014-
dc.identifier.isiWOS:000343210300032-
dc.identifier.scopus2-s2.0-84907895408-
dc.identifier.pmid25310818-
dc.identifier.eissn1932-6203en_US
dc.identifier.rosgroupid2014002175-
dc.description.ros2014-2015 > Academic research: refereed > Publication in refereed journalen_US
dc.description.validate201811_a bcmaen_US
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRAen_US
dc.description.pubStatusPublisheden_US
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