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Title: Determination of exogenous epigallocatechin gallate peracetate in mouse plasma using liquid chromatography with quadrupole time-of-flight mass spectrometry
Authors: Chu, KO
Man, GCW
Chan, KP
Chu, CY
Chan, TH 
Pang, CP
Wang, CC
Keywords: Epigallocatechin gallate peracetate
Liquid chromatography
Mass spectrometry
Plasma profile
Issue Date: 2014
Publisher: Wiley-VCH
Source: Journal of separation science, 2014, v. 37, no. 23, p. 3473-3480 How to cite?
Journal: Journal of separation science 
Abstract: A robust method for the quantitation of epigallocatechin gallate peracetate in plasma for pharmacokinetic studies is lacking. We have developed a validated method to quantify this compound using liquid chromatography with quadrupole time-of-flight mass spectrometry with isopropanol and tert-butylmethyl ether (3: 10) extraction and thin-layer chromatography purification. The epigallocatechin gallate peracetate-1-C-13(8) isotope was used as an internal standard. The linear range (r(2) > 0.9950) was from 0.05 to 100.00 mu g/mL. The lower limit of quantification of the method was 0.05 mu g/mL. Reproducibility, coefficient of variation, was between 0.7 and 12.6% (n = 6), accuracy between 83.7 and 104.6% (n = 5), and recovery ranged from 82.4 to 109.0% (n = 4). Ion suppression was approximately 40%. No mass spectral peaks were found to interfere between the standard and internal standard or the blank plasma extracts. Epigallocatechin gallate peracetate in plasma was stably stored at -80 degrees C over three months even after three freeze-thaw cycles. Extracts were stable in the sampler at 4 degrees C for over 48 h. Plasma levels weremaintained at 1.36 mu g/mL for 360 min after intraorbital intravenous injection at 50 mg/kg in mice. This method can be used to reliably measure epigallocatechin gallate peracetate in plasma for pharmacokinetic studies.
ISSN: 1615-9306
EISSN: 1615-9314
DOI: 10.1002/jssc.201400701
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