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Title: Epigenetic changes of TIMP-3, GSTP-1 and 14-3-3 sigma genes as indication of status of chronic inflammation and cancer
Authors: Wang, YJ
He, L
Yuan, M
Tsang, WWN 
Hao, L
Wang, M
Chow, LWC
Cheung, MNB
Liu, Q
Loo, WTY
Chow, CYC
Bai, LJ
Yang, Z
Keywords: 14-3-3 sigma
Breast cancer
Chronic periodontitis
DNA methylation
Epigenetic changes
Issue Date: 2014
Publisher: Wichtig Editore
Source: International journal of biological markers, 2014, v. 29, no. 3, p. E208-E214 How to cite?
Journal: International journal of biological markers 
Abstract: Objectives: This study aimed to compare the epigenetic changes via hypermethylation status of TIMP-3, GSTP-1 and 14-3-3 sigma genes, between healthy subjects and patients with reversible chronic inflammatory disease, and between healthy subjects and patients with irreversible malignant disease, to highlight the genetic changes that occur in the progression from an inflammatory condition to irreversible genetic changes commonly observed in cancer patients.
Methods: DNA was extracted from the blood of 680 healthy subjects, and tissues and blood of 110 patients with chronic inflammation disease of the gums, as well as neoplastic tissues of 108 breast cancer patients. Methylation-specific polymerase chain reaction (PCR) for TIMP-3, GSTP-1 and 14-3-3 sigma was performed, and hypermethylation status was analyzed and compared between the 3 groups.
Results: The hypermethylation frequencies of TIMP-3 and GSTP-1 of reversible chronic inflammatory gum disease and the control group were similar, but both were significantly lower than those for malignant disease patients (p<0.0001). The methylation frequency of 14-3-3 sigma in chronic inflammatory gum disease was higher than in the cancer and control groups (p<0.0001). The methylation of CpG islands in TIMP-3 and GSTP-1 in chronic inflammation patients occurred as frequently as in the control group, but less frequently than in breast cancer patients. However, the epigenetic silencing of 14-3-3 sigma occurred more frequently in the chronic inflammation group than in cancer patients and healthy controls.
Conclusions: The epigenetic silencing of 14-3-3 sigma might be essential for chronic inflammatory gum disease. The epigenetic changes presented in chronic inflammation patients might demonstrate an irreversible destruction in the tissues or organs similar to cancer.
ISSN: 0393-6155
DOI: 10.5301/jbm.5000104
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