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Title: An antioxidative galactomannan-protein complex isolated from fermentation broth of a medicinal fungus Cs-HK1
Authors: Chen, X
Ding, ZY
Wang, WQ
Siu, KC
Wu, JY 
Keywords: Cordyceps sinensis
Polysaccharide-protein complex
Issue Date: 2014
Publisher: Elsevier
Source: Carbohydrate polymers, 2014, v. 112, p. 469-474 How to cite?
Journal: Carbohydrate polymers 
Abstract: A protein-containing polysaccharide, EPS2BW, was fractionated from the exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis (Cs-HK1). EPS2BW was mainly composed of galactomannan with about 16% (w/w) protein and 50 kDa average molecular weight. The galactomannan part consisted of mannose and galactose at 1.7:1.0 molar ratio, and the protein segments were composed of sixteen amino acids with 12.5% proline and 16.6% threonine (mol%) being the most abundant. Based on analytical results from NMR, methylation analysis, partial acid hydrolysis and GC-MS, the galactomannan structure was elucidated as a (1 -> 2)-alpha-D-mannopyranosyl (Manp) backbone with 0-6-linked galactopyranosyl (Galp) branches. EPS2BW exhibited a high antioxidant capacity in both chemical and cell culture assays, with a Trolox equivalent radical scavenging activity of 44.7 mu mol Trolox/mg, a Fe3+ reducing power of 38.9 mu mol Fe2+/mg, and significant cytoprotective effect against H2O2-induced PC12 cell death at 50-250 mu g/mL.
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2014.06.021
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