Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/35570
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Guo, J | - |
dc.creator | Xu, C | - |
dc.creator | Li, X | - |
dc.creator | Chen, S | - |
dc.date.accessioned | 2016-04-15T08:34:14Z | - |
dc.date.available | 2016-04-15T08:34:14Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/35570 | - |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | © 2014 Guo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.rights | The following publication: Guo J, Xu C, Li X, Chen S (2014) A Simple, Rapid and Sensitive FRET Assay for Botulinum Neurotoxin Serotype B Detection. PLoS ONE 9(12): e114124 is available at https://doi.org/10.1371/journal.pone.0114124 | en_US |
dc.title | A simple, rapid and sensitive FRET assay for botulinum neurotoxin serotype B detection | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.doi | 10.1371/journal.pone.0114124 | en_US |
dcterms.abstract | Botulinum neurotoxins (BoNTs), the most potent naturally-occurring neurotoxins known to humans, comprise seven distinct serotypes (BoNT/A-G), each of which exhibits unique substrate specificity. Many methods have been developed for BoNT detection, in particular for BoNT/A, with various complexity and sensitivity, while substrate based FRET assay is considered as the most widely used approach due to its simplicity and sensitivity. In this study, we designed a vesicle-associated membrane protein 2 (VAMP2) based FRET assay based on the understanding of the VAMP2 and light chain/B (LC/B) interactions in our previous studies. The current design constituted the shortest peptide, VAMP2 (63-85), with FRET dyes (EDAN and Dabcyl) labelled at position 76 and 85, respectively, which showed minimal effect on VAMP2 substrate catalysis by LC/B and therefore enhanced the sensitivity of the assay. The FRET peptide, designated as FVP-B, was specific to LC/B, with a detection sensitivity as low as ∼20 pM in 2 h. Importantly, FVP-B showed the potential to be scaled up and used in high throughput screening of LC/B inhibitor. The currently developed FRET assay is one of the most economic and rapid FRET assays for LC/B detection. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | PLoS one, 2014, v. 9, no. 12, e114124 | - |
dcterms.isPartOf | PLoS one | - |
dcterms.issued | 2014 | - |
dc.identifier.scopus | 2-s2.0-84914689164 | - |
dc.identifier.pmid | 25437190 | - |
dc.identifier.eissn | 1932-6203 | en_US |
dc.identifier.rosgroupid | 2014004349 | - |
dc.description.ros | 2014-2015 > Academic research: refereed > Publication in refereed journal | en_US |
dc.description.validate | 201810_a bcma | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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Guo_simple_rapid_sensitive.PDF | 770.52 kB | Adobe PDF | View/Open |
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