Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/35570
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dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorGuo, J-
dc.creatorXu, C-
dc.creatorLi, X-
dc.creatorChen, S-
dc.date.accessioned2016-04-15T08:34:14Z-
dc.date.available2016-04-15T08:34:14Z-
dc.identifier.urihttp://hdl.handle.net/10397/35570-
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rights© 2014 Guo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rightsThe following publication: Guo J, Xu C, Li X, Chen S (2014) A Simple, Rapid and Sensitive FRET Assay for Botulinum Neurotoxin Serotype B Detection. PLoS ONE 9(12): e114124 is available at https://doi.org/10.1371/journal.pone.0114124en_US
dc.titleA simple, rapid and sensitive FRET assay for botulinum neurotoxin serotype B detectionen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume9en_US
dc.identifier.issue12en_US
dc.identifier.doi10.1371/journal.pone.0114124en_US
dcterms.abstractBotulinum neurotoxins (BoNTs), the most potent naturally-occurring neurotoxins known to humans, comprise seven distinct serotypes (BoNT/A-G), each of which exhibits unique substrate specificity. Many methods have been developed for BoNT detection, in particular for BoNT/A, with various complexity and sensitivity, while substrate based FRET assay is considered as the most widely used approach due to its simplicity and sensitivity. In this study, we designed a vesicle-associated membrane protein 2 (VAMP2) based FRET assay based on the understanding of the VAMP2 and light chain/B (LC/B) interactions in our previous studies. The current design constituted the shortest peptide, VAMP2 (63-85), with FRET dyes (EDAN and Dabcyl) labelled at position 76 and 85, respectively, which showed minimal effect on VAMP2 substrate catalysis by LC/B and therefore enhanced the sensitivity of the assay. The FRET peptide, designated as FVP-B, was specific to LC/B, with a detection sensitivity as low as ∼20 pM in 2 h. Importantly, FVP-B showed the potential to be scaled up and used in high throughput screening of LC/B inhibitor. The currently developed FRET assay is one of the most economic and rapid FRET assays for LC/B detection.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationPLoS one, 2014, v. 9, no. 12, e114124-
dcterms.isPartOfPLoS one-
dcterms.issued2014-
dc.identifier.scopus2-s2.0-84914689164-
dc.identifier.pmid25437190-
dc.identifier.eissn1932-6203en_US
dc.identifier.rosgroupid2014004349-
dc.description.ros2014-2015 > Academic research: refereed > Publication in refereed journalen_US
dc.description.validate201810_a bcmaen_US
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRAen_US
dc.description.pubStatusPublisheden_US
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