Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/35492
Title: A graphene oxide based fluorescence resonance energy transfer (FRET) biosensor for ultrasensitive detection of botulinum neurotoxin A (BoNT/A) enzymatic activity
Authors: Shi, J
Guo, J
Bai, G
Chan, C
Liu, X
Ye, W
Hao, J 
Chen, S 
Yang, M 
Keywords: Botulinum neurotoxins (BoNTs)
Graphene oxide (GO)
Green fluorescence protein (GFP)
Optical detection
SNAP-25
Issue Date: 2015
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2015, v. 65, p. 238-244 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: Botulinum neurotoxins (BoNTs) are among the most potent toxic bacterial proteins for humans, which make them potential agents for bioterrorism. Therefore, an ultrasensitive detection of BoNTs and their active states is in great need as field-deployable systems for anti-terrorism applications. We report the construction of a novel graphene oxide (GO)-peptide based fluorescence resonance energy transfer (FRET) biosensor for ultrasensitive detection of the BoNT serotype A light chain (BoNT-LcA) protease activity. A green fluorescence protein (GFP) modified SNAP-25 peptide substrate (SNAP-25-GFP) was optimally designed and synthesized with the centralized recognition/cleavage sites. This FRET platform was constructed by covalent immobilization of peptide substrate on GO with BSA passivation which have advantages of low non-specific adsorption and high stability in protein abundant solution. BoNT-LcA can specifically cleave SNAP-25-GFP substrate covalently immobilized on GO to release the fragment with GFP. Based on fluorescence signal recovery measurement, the target BoNT-LcA was detected sensitively and selectively with the linear detection range from 1. fg/mL to 1. pg/mL. The limit of detection (LOD) for BoNT-LcA is around 1. fg/mL.
URI: http://hdl.handle.net/10397/35492
ISSN: 0956-5663 (print)
1873-4235 (online)
DOI: 10.1016/j.bios.2014.10.050
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

14
Last Week
1
Last month
Citations as of Mar 18, 2017

WEB OF SCIENCETM
Citations

12
Last Week
0
Last month
Citations as of Mar 17, 2017

Page view(s)

22
Last Week
3
Last month
Checked on Mar 19, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.