Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/34949
Title: Phyllanthus urinaria extract attenuates acetaminophen induced hepatotoxicity : involvement of cytochrome P450 CYP2E1
Authors: Hau, DKP
Gambari, R
Wong, RSM
Yuen, MCW
Cheng, GYM
Tong, CSW
Zhu, GY
Leung, AKM
Lai, PBS
Lau, FY
Chan, AKW
Wong, WY
Kok, SHL
Cheng, CH
Kan, CW
Chan, ASC
Chui, CH
Tang, JCO 
Fong, DWF
Keywords: Acetaminophen
Cytochrome 450 CYP2E1
Hepatoprotection
Hepatotoxicity
Phyllanthus urinaria
Issue Date: 2009
Publisher: Elsevier
Source: Phytomedicine, 2009, v. 16, no. 8, p. 751-760 How to cite?
Journal: Phytomedicine
Abstract: Acetaminophen is a commonly used drug for the treatment of patients with common cold and influenza. However, an overdose of acetaminophen may be fatal. In this study we investigated whether mice, administered intraperitoneally with a lethal dose of acetaminophen, when followed by oral administration of Phyllanthus urinaria extract, may be prevented from death. Histopathological analysis of mouse liver sections showed that Phyllanthus urinaria extract may protect the hepatocytes from acetaminophen-induced necrosis. Therapeutic dose of Phyllanthus urinaria extract did not show any toxicological phenomenon on mice. Immunohistochemical staining with the cytochrome P450 CYP2E1 antibody revealed that Phyllanthus urinaria extract reduced the cytochrome P450 CYP2E1 protein level in mice pre-treated with a lethal dose of acetaminophen. Phyllanthus urinaria extract also inhibited the cytochrome P450 CYP2E1 enzymatic activity in vitro. Heavy metals, including arsenic, cadmium, mercury and lead, as well as herbicide residues were not found above their detection limits. High performance liquid chromatography identified corilagin and gallic acid as the major components of the Phyllanthus urinaria extract. We conclude that Phyllanthus urinaria extract is effective in attenuating the acetaminophen induced hepatotoxicity, and inhibition of cytochrome P450 CYP2E1 enzyme may be an important factor for its therapeutic mechanism.
URI: http://hdl.handle.net/10397/34949
ISSN: 0944-7113
DOI: 10.1016/j.phymed.2009.01.008
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