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Title: Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A
Authors: Ye, W
Guo, J
Chen, S 
Yang, M 
Issue Date: 2013
Publisher: Royal Society of Chemistry
Source: Journal of materials chemistry B, 2013, v. 1, no. 47, p. 6544-6550 How to cite?
Journal: Journal of materials chemistry B
Abstract: Botulinum neurotoxins are among the most potent toxic bacterial proteins for humans and there is a great need to develop simple, rapid and sensitive methods for toxin detection and protease activity quantification in field deployment. In this paper, a nanoporous membrane based impedance sensor was developed to monitor the activity of the BoNT serotype A light chain protease (LcA). Synaptosomal-associated protein 25 (SNAP-25) was first immobilized inside nanopore walls via silane linkers. BoNT LcA was then injected over the nanoporous membrane substrate sensor and specifically cleaved SNAP-25. The cleavage activity could be monitored by measuring impedance signals across nanoporous membranes which represented the nanopore blockage degree. This initial device could achieve a 500 pM LcA detection limit within 25 minutes.
ISSN: 2050-750X (print)
DOI: 10.1039/C3TB21152E
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