Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/34314
Title: L2DTL/CDT2 and PCNA interact with p53 and regulate p53 polyubiquitination and protein stability through MDM2 and CUL4A/DDB1 complexes
Authors: Banks, D
Wu, M
Higa, LA
Gavrilova, N
Quan, J
Ye, T 
Kobayashi, R
Sun, H
Zhang, H
Keywords: CUL4
DDB1
p53
MDM2/HDM2
PCNA,L2DTL/CDT2
Proteolysis
Issue Date: 2006
Publisher: Taylor & Francis
Source: Cell cycle, 2006, v. 5, no. 15, p. 1719-1729 How to cite?
Journal: Cell cycle 
Abstract: The CUL4-ROC1 E3 ligase complex regulates genome stability, replication, and cell cycle progression. A novel WD40 domain-containing protein, L2DTL, and PCNA were identified as proteins associated with CUL4/DDB1 complexes. Inactivation of CUL4A, L2DTL, PCNA, DDB1, or ROC1 induced p53 stabilization and growth arrest. L2DTL, PCNA, and DDB1/CUL4A complexes were found to physically interact with p53 tumor suppressor and its regulator MDM2/HDM2. The isolated CUL4A complexes display potent and robust polyubiquitination activity towards p53 and this activity is dependent on L2DTL, PCNA, DDB1, ROC1, and MDM2/HDM2. We also found that the interaction between p53 and CUL4 complex is regulated by DNA damage. Our data further showed that MDM2/HDM2 is rapidly proteolyzed in response to UV irradiation and this process is regulated by CUL4/DDB1 and PCNA. Our studies demonstrate that PCNA, L2DTL, and the DDB1-CUL4A complex play critical and differential roles in regulating the protein stability of p53 and MDM2/HDM2 in unstressed and stressed cells.
URI: http://hdl.handle.net/10397/34314
ISSN: 1538-4101
DOI: 10.4161/cc.5.15.3150
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