Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/34106
Title: A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus
Authors: Shi, J
Chan, C
Pang, Y
Ye, W
Tian, F
Lyu, J
Zhang, Y
Yang, M 
Keywords: Fluorescence resonance energy transfer (FRET)
Foodborne bacteria
Gold nanoparticles (AuNPs)
Graphene quantum dots (GQDs)
Issue Date: 2015
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2015, v. 67, p. 595-600 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100. nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1. nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor.
URI: http://hdl.handle.net/10397/34106
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/j.bios.2014.09.059
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