Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/34049
Title: A simple, highly sensitive, high throughput and organic solvent-free screening method for melamine by microsphere-based flow cytometry immunoassay
Authors: Tsoi, TH
Wong, WT 
Issue Date: 2015
Publisher: Royal Society of Chemistry
Source: Analytical methods, 2015, v. 7, no. 14, p. 5989-5995 How to cite?
Journal: Analytical Methods 
Abstract: In recent years, the safety problem of infant formula powder has provoked panic among Chinese citizens. One of the most notorious incidents was the 2008 Chinese milk scandal which harmed thousands of infants. Therefore the development of a fast, high throughput screening method towards melamine and other harmful chemicals is of paramount importance. Microsphere-based flow cytometry is a new multiplexing immunochemical method for food-testing. Through competition between the free analyte in the sample and analyte-coupled microspheres, the content of the analyte can be indirectly quantified by measuring the fluorescence emitted from labelled antibodies. This developed method of high sensitivity (limit of detection = 0.70 ng ml-1) has already been validated and successfully applied to screen for melamine in various brands of infant formula powder analysis fulfilling the legislated tolerance level in a simple, fast, high throughput and organic solvent-free manner.
URI: http://hdl.handle.net/10397/34049
ISSN: 1759-9660
DOI: 10.1039/c5ay00648a
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

2
Last Week
0
Last month
0
Citations as of Nov 30, 2017

WEB OF SCIENCETM
Citations

2
Last Week
0
Last month
0
Citations as of Dec 3, 2017

Page view(s)

88
Last Week
4
Last month
Checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.