Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/33604
Title: Gleditsia sinensis fruit extract is a potential chemotherapeutic agent in chronic and acute myelogenous leukemia
Authors: Chow, LMC 
Chui, CH
Tang, JCO 
Teo, ITN
Lau, FY
Cheng, GYM
Wong, RSM
Leung, TWT
Lai, KB
Yau, MYC
Issue Date: 2003
Publisher: Spandidos Publications
Source: Oncology reports, 2003, v. 10, no. 5, p. 1601-1607 How to cite?
Journal: Oncology reports 
Abstract: The anti-leukemia activity of the saponin rich Gleditsia sinensis Lam. fruit extract (GSE) was investigated on cancer cell lines and bone marrow cells obtained from consented patients with chronic myelogenous leukemia (CML) and acute myelogenous leukemia (AML) during presentation. The growth inhibitory activity of the extract was determined by [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) assay. Colony formation assay was performed to investigate the regeneration potential. Cellular morphology change was studied. Apoptosis was demonstrated by DNA electrophoresis, reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry. The mean concentration to inhibit the cell growth by 50% (MTS50) was 18±1.6 µg/ml for K562 CML cell line and 12±1.3 µg/ml for HL-60 acute promyelocytic leukemia cell line. Patient samples showed a mean MTS50 of 13-28 µg/ml. Non-malignant hematological disorder bone marrow samples showed a mean MTS50 from 45 to 53 µg/ml. Loss of regeneration property after treatment with GSE of these two cancer cell lines were confirmed by colony formation assay. GSE was able to induce cell shrinkage in K-562. DNA laddering was observed by incubating the leukemia cells with GSE. RT-PCR demonstrated that the pro-apoptic gene bax was induced while the anti-apoptic gene bcl-2 and cell cycle active gene PCNA were reduced. Flow cytometric analysis showed that the apoptotic effect of GSE on leukemia cell line was time- and dose-dependent. Thus GSE might be potentially used as a chemotherapeutic drug to treat patients with acute and chronic myelogenous leukemia.
URI: http://hdl.handle.net/10397/33604
ISSN: 1021-335X
EISSN: 1791-2431
DOI: 10.3892/or.10.5.1601
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

Page view(s)

39
Last Week
1
Last month
Checked on Aug 20, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.