Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/33154
Title: Single-stranded DNA concentration by electrokinetic forces
Authors: Cheng, H
Lei, KF
Choy, KY
Chow, LMC 
Keywords: Electrodes
Molecules
Numerical simulations
Gold
Microscopes
Glasses
Issue Date: 2009
Publisher: Society of Photo-Optical Instrumentation Engineers
Source: Journal of micro/nanolithography MEMS and MOEMS, 2009, v. 8, no. 2, 021107 How to cite?
Journal: Journal of micro/nanolithography MEMS and MOEMS 
Abstract: Electrokinetics provide an effective molecular manipulation technique in micro/nano fluidic environments, which match the length scale of various biological objects. In this work, two circular gold electrodes are fabricated on a glass substrate as DNA concentrators. A combination of alternating current (ac) electro-osmosis and electrophoresis is used to concentrate single-stranded DNA (ssDNA) molecules as small as 20 nucleotides in length. To understand the phenomenon of ac electro-osmotic flow, numerical simulation of the flow field is done and shows that the effective fluid flow is up to 100£gm above the electrode surface. Hence, ssDNA molecules labeled with fluorescent dye are utilized to demonstrate the concentration effect on the DNA concentrator. ac electro-osmotic flow induced by the ac electric field can stir the bulk fluid, and ssDNA molecules can be transported from a large effective region to the surface of DNA concentrator. In addition, electrophoretic
URI: http://hdl.handle.net/10397/33154
ISSN: 1932-5150
DOI: 10.1117/1.3152003
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