Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/33106
Title: Monolithic silica columns with mixed mode of hydrophilic interaction and weak anion-exchange stationary phase for pressurized capillary electrochromatography
Authors: Ye, F
Xie, Z
Wong, KY 
Keywords: Hydrophilic interaction
Monolithic column
Pressurized capillary electro-chromatography
Sol-gel
Stationary phase
Issue Date: 2006
Source: Electrophoresis, 2006, v. 27, no. 17, p. 3373-3380 How to cite?
Journal: Electrophoresis 
Abstract: A silica-based monolithic column as polar stationary phase is proposed for pressurized CEC (pCEC). The monolithic silica matrix from a sol-gel process was chemically modified by 3-aminopropyltrimethoxysilane to produce a column for hydrophilic interaction applications. The amino groups on the surface of the polar stationary phase generated anodic EOF under acidic conditions and served at the same time as a weak anion-exchanger. The anion solutes such as nucleotides were separated by the mixed mode mechanism, which comprised hydrophilic interaction, weak anion-exchange, and electrophoresis. The influences of buffer concentration and organic modifier content on the separation of nucleotides by pCEC have been investigated. In addition, the monolithic silica columns were also able to separate various polar compounds such as phenols, nucleic acid bases, and nucleosides in the hydrophilic interaction CIEC mode.
URI: http://hdl.handle.net/10397/33106
ISSN: 0173-0835
DOI: 10.1002/elps.200500869
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