Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/32751
Title: Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates
Authors: Wang, Y
Ruan, L
Lo, WH 
Chua, H
Yu, HF
Keywords: Bacillus subtilis
Cloning and expression
P (HB-co-mclHA)
PHA synthase gene
Polyhydroxyalkanoates (PHAs)
Issue Date: 2006
Publisher: Humana Press
Source: Applied biochemistry and biotechnology, 2006, v. 132, no. 1-3, p. 1015-1022 How to cite?
Journal: Applied biochemistry and biotechnology 
Abstract: Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1 and pBE2C1AB, containing one or two PHA synthse genes, respectively. The two plasmids were inserted into Bacillus subtilis DB104 to generate modified strains, B. subtilis/pBE2C1 and B. subtilis/pBE2C1AB. The two recombinants strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in B. subtilis. Gas Chromatography analysis identified the compound produced by B. subtilis/pBE2C1 to be a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer whereas that produced by B. subtilis/pBE2C1AB was a hydroxybutyrate-co- hydroxydecanoate-co-hydroxydodecanoate (HB-HD-HDD) polymer.
URI: http://hdl.handle.net/10397/32751
ISSN: 0273-2289
EISSN: 1559-0291
DOI: 10.1385/ABAB:132:1:1015
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