Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/32581
Title: Endothelial nitric oxide synthase is a critical factor in experimental liver fibrosis
Authors: Leung, TM
Tipoe, GL
Liong, EC
Lau, TYH
Fung, ML
Nanji, AA
Issue Date: 2008
Source: International journal of experimental pathology, 2008, v. 89, no. 4, p. 241-250
Abstract: Reduced expression of endothelial nitric oxide synthase (eNOS) in chronic liver disease can reduce hepatic perfusion and accelerate fibrosis. The relationship between eNOS expression and liver fibrogenesis remains unclear. We investigated whether l-arginine attenuated chronic liver fibrosis through eNOS expression. Chronic liver injury was induced by administration of carbon tetrachloride (CCl 4) to mice for 8 weeks. 5-Methylisothiourea hemisulphate (SMT), an iNOS inhibitor, or l-arginine, a NOS substrate were injected subcutaneously. CCl 4-induced hepatotoxicity, oxidative stress and accumulation of collagen were detected in the liver. The expression levels of inducible NOS (iNOS) and nuclear factor kappa-B (NF-κB) activity in the liver after CCl 4 treatment were increased but eNOS expression and activator protein-1 (AP-1) activity were decreased. Both SMT and l-arginine effectively reduced CCl 4 induced oxidative stress and collagen formation, but l-arginine showed a significantly greater suppression of collagen formation, iNOS expression and NF-κB activity. l-Arginine also restored the level of eNOS and AP-1 activity. l-Arginine was more effective than SMT in suppressing liver fibrosis. l-Arginine might improve NO production which facilitates hepatic blood flow and thus retards liver fibrogenesis. Our results showed that the reduced eNOS expression in CCl 4-treated mice was reversed by l-arginine. Furthermore, l-arginine also reversed the reduced AP-1 activity, an eNOS promoter.
Keywords: Carbon tetrachloride
Chronic liver injury
L-arginine
Nitric oxide synthase
Publisher: Wiley-Blackwell
Journal: International Journal of Experimental Pathology 
DOI: 10.1111/j.1365-2613.2008.00590.x
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

29
Last Week
0
Last month
0
Citations as of Sep 5, 2020

WEB OF SCIENCETM
Citations

32
Last Week
0
Last month
0
Citations as of Sep 25, 2020

Page view(s)

115
Last Week
0
Last month
Citations as of Sep 23, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.