Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/31841
Title: Styryl quinolinium/G-quadruplex complex for dual-channel fluorescent sensing of Ag + and cysteine
Authors: Lu, YJ
Ma, N
Li, YJ
Lin, ZY
Qiu, B
Chen, GN
Wong, KY 
Keywords: Ag +
Cysteine
Dual-channel
Fluorescent sensing
G-quadruplex
Styryl quinolinium (SQ)
Issue Date: 2012
Publisher: Elsevier
Source: Sensors and actuators. B, Chemical, 2012, v. 173, p. 295-299 How to cite?
Journal: Sensors and actuators. B, Chemical 
Abstract: Based on the disruption and recovery of fluorescence of styryl quinolinium (SQ) dye/G-quadruplex complex, a label-free method for dual-channel detection of Ag + and cysteine is developed. The above sensing system exhibits high sensitivity and good selectivity for Ag +, even in the presence of other metal ions (such as Na +, Mg 2+, Mn 2+, Ba 2+, Ca 2+, Pb 2+, Zn 2+, Cd 2+, Hg 2+ and Cu 2+). Due to cysteine (Cys) is a strong Ag +-binder and competes with quadruplex-forming G-rich oligonucleotides for Ag + binding, and results in the reformation of G-quadruplex structure and the recovery of fluorescence. Thus SQ dye/G-quadruplex complexes are not only a potential primary sensor toward Ag +, but also a secondary sensor toward Cys. Neither the Ag +-sensing nor the Cys-sensing systems required labeled oligonucleotides. Under the optimal conditions, this method is capable of detection of Ag + in the range of 0.1-15.0 μM with a detection limit of 26.0 nM. In addition, the proposed method allows a good response to cysteine with a linear range of 0.008-10 μM, and the limit of detection is found to be 2.0 nM. Moreover, this method has been applied successfully to the sensing of cysteine in human urine with satisfactory recovery.
URI: http://hdl.handle.net/10397/31841
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2012.07.007
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