Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/31830
Title: Z-ligustilide potentiates the cytotoxicity of dopamine in rat dopaminergic PC12 cells
Authors: Qi, H
Zhao, J
Han, Y 
Lau, ASY
Rong, J
Keywords: Cytotoxicity
Dopamine
Parkinson's disease
Reactive oxygen species
Reduced glutathione
Z-Ligustilide
Issue Date: 2012
Publisher: Springer
Source: Neurotoxicity research, 2012, v. 22, no. 4, p. 345-354 How to cite?
Journal: Neurotoxicity Research 
Abstract: Abstract Dopamine toxicity is an ongoing controversy surrounding the use of levadopa (L-Dopa) in the therapy of Parkinson's disease. The initial objective of this study was to investigate the potential of neuroprotective botanicals such as Z-ligustilide in reducing the cytotoxicity of dopamine. We surprisingly found that Z-ligustilide potentiated dopamine toxicity in a dopaminergic cell specific manner. Using rat dopaminergic cell line PC12 as a model, we demonstrated that dopamine and Z-ligustilide in combination profoundly induced cell death, although these drugs alone, to a lesser extent, affected the cell viability in a concentration-dependent manner. The synergistic cytotoxicity of dopamine and Z-ligustilide is likely mediated via apoptosis, characterized by DNA fragmentation and chromatin shrinking after 12 h incubation. By measuring the intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), Z-ligustilide and dopamine in combination dramatically enhanced the ROS formation and further depleted reduced GSH, whereas these drugs alone showed much less activity. Importantly, the synergistic cytotoxicity of dopamine and Z-ligustilide could be largely prevented by thiol-containing antioxidant N-acetylcysteine and GSH other than vitamin C and Trolox. Since the cytotoxicity of Z-ligustilide was not reported previously, the results of this study should raise public concerns over the potential risk associated with the combined use of herbal medicines containing Z-ligustilide with L-Dopa in the therapy of Parkinson's disease.
URI: http://hdl.handle.net/10397/31830
DOI: 10.1007/s12640-012-9319-6
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

9
Last Week
0
Last month
1
Citations as of Dec 3, 2017

WEB OF SCIENCETM
Citations

7
Last Week
0
Last month
1
Citations as of Nov 15, 2017

Page view(s)

59
Last Week
4
Last month
Checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.