Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/31579
DC FieldValueLanguage
dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorZou, L-
dc.creatorPang, HL-
dc.creatorChan, PH-
dc.creatorHuang, ZS-
dc.creatorGu, LQ-
dc.creatorWong, KY-
dc.date.accessioned2014-12-19T07:02:32Z-
dc.date.available2014-12-19T07:02:32Z-
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/10397/31579-
dc.language.isoenen_US
dc.titleCovalent immobilization of carbohydrates on sol-gel-coated microplatesen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage1195-
dc.identifier.epage1200-
dc.identifier.volume133-
dc.identifier.issue9-
dc.identifier.doi10.1039/b805346d-
dcterms.abstractCarbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein-carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol-gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact carbohydrates on sol-gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein-carbohydrate interactions in a high-throughput manner.-
dcterms.bibliographicCitationAnalyst, 2008, v. 133, no. 9, p. 1195-1200-
dcterms.isPartOfAnalyst-
dcterms.issued2008-
dc.identifier.isiWOS:000259449400011-
dc.identifier.scopus2-s2.0-49849098689-
dc.identifier.pmid18709194-
dc.identifier.rosgroupidr43565-
dc.description.ros2008-2009 > Academic research: refereed > Publication in refereed journal-
Appears in Collections:Journal/Magazine Article
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

SCOPUSTM   
Citations

4
Last Week
0
Last month
0
Citations as of Jun 30, 2020

WEB OF SCIENCETM
Citations

4
Last Week
0
Last month
0
Citations as of Jul 9, 2020

Page view(s)

102
Last Week
1
Last month
Citations as of Feb 17, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.