Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/31579
Title: Covalent immobilization of carbohydrates on sol-gel-coated microplates
Authors: Zou, L
Pang, HL
Chan, PH 
Huang, ZS
Gu, LQ
Wong, KY 
Issue Date: 2008
Source: Analyst, 2008, v. 133, no. 9, p. 1195-1200 How to cite?
Journal: Analyst 
Abstract: Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein-carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol-gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact carbohydrates on sol-gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein-carbohydrate interactions in a high-throughput manner.
URI: http://hdl.handle.net/10397/31579
ISSN: 0003-2654
DOI: 10.1039/b805346d
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