Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/31386
DC FieldValueLanguage
dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorSiu, FM-
dc.creatorMa, DL-
dc.creatorCheung, YW-
dc.creatorLok, CN-
dc.creatorYan, K-
dc.creatorYang, Z-
dc.creatorYang, M-
dc.creatorXu, S-
dc.creatorKo, BCB-
dc.creatorHe, QY-
dc.creatorChe, CM-
dc.date.accessioned2015-06-23T09:07:03Z-
dc.date.available2015-06-23T09:07:03Z-
dc.identifier.issn1615-9853-
dc.identifier.urihttp://hdl.handle.net/10397/31386-
dc.language.isoenen_US
dc.publisherWiley-VCHen_US
dc.subjectApoptosisen_US
dc.subjectLung canceren_US
dc.subjectProteomicsen_US
dc.subjectSaponinen_US
dc.subjectTranscriptomicsen_US
dc.titleProteomic and transcriptomic study on the action of a cytotoxic saponin (Polyphyllin D) : induction of endoplasmic reticulum stress and mitochondria-mediated apoptotic pathwaysen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage3105-
dc.identifier.epage3117-
dc.identifier.volume8-
dc.identifier.issue15-
dc.identifier.doi10.1002/pmic.200700829-
dcterms.abstractPolyphyllin D (PD) is a potent cytotoxic saponin found in Paris polyphylla. In the present study, bioinformatic, proteomic and transcriptomic analyses were performed to study the mechanisms of action of PD on human nonsmall cell lung cancer (NSCLC) cell line (NCI-H460). Using a gene expression-based bioinformatic tool (connectivity map), PD was identified as a potential ER stress inducer. Our proteomic and transcriptomic analyses revealed that PD treatment led to upregulation of typical ER stress-related proteins/genes including glucose-regulated protein 78 (BiP/GRP78) and protein disulfide isomerase (PDI). In particular, elevated expression of C/EBP homologous transcription factor (chop) and activation of caspase-4 occurred at early time point (8 h) of PD treatment, signifying an initial ER stress-mediated apoptosis. Induction of tumor suppressor p53, disruption of mitochondrial membrane, activation of caspase-9 and caspase-3 were detected upon prolonged PD treatment. Collectively, these data revealed that PD induced the cytotoxic effect through a mechanism initiated by ER stress followed by mitochondrial apoptotic pathway. The ability of activating two major pathways of apoptosis makes PD an attractive drug lead for anticancer therapeutics.-
dcterms.bibliographicCitationProteomics, 2008, v. 8, no. 15, p. 3105-3117-
dcterms.isPartOfProteomics-
dcterms.issued2008-
dc.identifier.isiWOS:000258503400012-
dc.identifier.scopus2-s2.0-49749104260-
dc.identifier.pmid18615425-
dc.identifier.eissn1615-9861-
dc.identifier.rosgroupidr41712-
dc.description.ros2008-2009 > Academic research: refereed > Publication in refereed journal-
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