Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30793
Title: Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha
Authors: Yan, Q
Sun, Y
Ruan, L
Chen, J
Yu, PHF
Keywords: Biosynthesis
Dual-nutrient-limitation zone
Ralstonia eutropha
scl-PHAs
Issue Date: 2005
Source: World journal of microbiology and biotechnology, 2005, v. 21, no. 1, p. 17-21 How to cite?
Journal: World Journal of Microbiology and Biotechnology 
Abstract: Biosynthesis of PHAs by Raltonia eutropha during the dual nutrient-limitation-zone was investigated with mixed organic acids as carbon sources and (NH4)2SO4 as nitrogen source. Two different methods of maintaining the dual-nutrient-limitation zone were adopted by feeding mixed acids and (NH4)2SO4 at determined rates into the fermentation cultures which were initially free of carbon sources (method A) or nitrogen sources (method B). The results indicate that, firstly, with the increase of the width of the dual-nutrient-limitation zone, the yield of short-chain-length-polyhydroxyalkanoates also increases and it suggests that most of the short-chain-length-polyhydroxyalkanoates were biosynthesized during the dual-nutrient-limitation zone. Secondly, in contrast with the dual-nutrient-limitation method of limiting the nitrogen source first (method B), the dual-nutrient-limitation method of limiting the carbon source first (method A) was more favourable for the production of short-chain-length- polyhydroxyalkanoates, and the maximum production of short-chain-length- polyhydroxyalkanoates of these two methods are 3.72 and 2.55 g/l, respectively.
URI: http://hdl.handle.net/10397/30793
ISSN: 0959-3993
DOI: 10.1007/s11274-004-0877-5
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