Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30787
Title: Oxidative burst, Jasmonic Acid Biosynthesis, and Taxol production induced by low-energy ultrasound in Taxus chinensis cell suspension cultures
Authors: Wu, J 
Ge, X
Keywords: Elicitation
Jasmonic acid
Reactive oxygen species
Taxol accumulation
Taxus chinensis cell
Ultrasound
Issue Date: 2004
Publisher: John Wiley & Sons
Source: Biotechnology and bioengineering, 2004, v. 85, no. 7, p. 714-721 How to cite?
Journal: Biotechnology and bioengineering 
Abstract: This work aims to detect the two signal events in the elicitation of plant defense responses and secondary metabolism in plant cell cultures by low-energy ultrasound (US), transient production of reactive oxygen species (ROS) or the oxidative burst and jasmonic acid (JA) biosynthesis, and examine their influence on secondary metabolism. Experiments were carried out in Taxus chinensis cell suspension culture which produces the anticancer diterpenoid Taxol (paclitaxel). The culture was exposed to low-frequency US for a short period of time (2 min). At sufficiently high US power levels the US exposure significantly enhanced the Taxol production and slightly depressed cell growth and viability. The US exposure induced transient production of O 2.- and H2O2 and an increase in the intracellular JA level as well as the activities of enzymes for JA synthesis, lipoxygenase (LOX), and allene oxide synthase (AOS). Inhibition of the ROS production by putative ROS scavengers or the JA accumulation by LOX inhibitors effectively suppressed the US-stimulated Taxol production. Inhibition of the ROS production also suppressed the US-induced JA accumulation. These results suggest that oxidative burst is an upstream event to JA accumulation, and both ROS from the oxidative burst and JA from the LOX pathway are key signal elements in the elicitation of Taxol production of T. chinensis cells by low-energy US.
URI: http://hdl.handle.net/10397/30787
ISSN: 0006-3592
DOI: 10.1002/bit.10911
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