Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30449
Title: A luminescence-based scanning respirometer for heavy metal toxicity monitoring
Authors: Wong, KY 
Zhang, MQ
Li, XM
Lo, W 
Keywords: Heavy metals
IC50
Luminescence quenching
Respirometer
Toxicity test
Issue Date: 1997
Publisher: Elsevier
Source: Biosensors and bioelectronics, 1997, v. 12, no. 2, p. 125-133 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+ and Hg2+ on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50 (concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.A respirometer has been developed for microbial toxicity tests. The measurement of oxygen concentration in the respirometer is based on luminescence quenching by molecular oxygen. The oxygen-sensing film consists of a luminescent ruthenium complex adsorbed on amorphous fumed silica and immobilized inside silicone rubber. The luminescence intensity depends on the oxygen concentration in the sample in contact with the sensing film. The inhibition effect of heavy metals on the respiration rate of microorganisms can be evaluated by monitoring the luminescence intensity of the sensing film. The toxicity of Cu2+, Zn2+, Ni2+, Cr3+, Pb2+ and Hg2+ on activated sludge was tested using the respirometer. The results indicated that the proposed method is a sensitive method for estimating IC50 (concentration of a toxicant producing 50% respiration inhibition) in microbial toxicity tests.
URI: http://hdl.handle.net/10397/30449
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/S0956-5663(97)87058-3
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