Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30418
Title: Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae
Authors: Hu, G
Siu, SO
Li, S
Chu, IK
Kwan, YW
Chan, SW
Leung, GPH
Yan, R
Lee, SMY
Keywords: Calycosin
Conjugation
Isoflavone
Metabolism
Zebrafish larvae
Issue Date: 2012
Publisher: Informa Healthcare
Source: Xenobiotica, 2012, v. 42, no. 3, p. 294-303 How to cite?
Journal: Xenobiotica 
Abstract: Although zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism.
URI: http://hdl.handle.net/10397/30418
DOI: 10.3109/00498254.2011.617015
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

21
Last Week
0
Last month
0
Citations as of Dec 3, 2017

WEB OF SCIENCETM
Citations

18
Last Week
1
Last month
0
Citations as of Dec 9, 2017

Page view(s)

51
Last Week
1
Last month
Checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.