Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30164
Title: Lntracellular Ca2+ regulates the cellular iron uptake in K562 cells
Authors: Ci, WM
Li, WY
Ke, Y
Qian, ZM
Shen, X
Keywords: Iron uptake
Transferrin receptor
Intracellular calcium
Endocytosis
Recycling
Actin polymerization
Issue Date: 2003
Publisher: Churchill Livingstone
Source: Cell calcium, 2003, v. 33, no. 4, p. 257-266 How to cite?
Journal: Cell Calcium 
Abstract: Fluorescence quenching was used to study the kinetics of the transferrin receptor (TfR)-mediated iron uptake in the calcein-loaded K562 cells. It was found that elevation of intracellular free Ca2+ ([Ca2+](i)) by thapsigargin (TG) speeds up the initial rate of iron uptake and increases the overall capacity of the cells in taking up iron. Depletion of intracellular Ca2+ or complete chelation of extracellular Ca2+ results in complete inhibition of the iron uptake in cells. To gain insight into molecular mechanism, IANBD-labeled transferrin (Tf) and microscopic fluorescence imaging were used to observe the endocytosis and recycling of the Tf-TfR complex in single live cells. The study showed that the preincubation of cells with TG or phorbol myristate acetate (PMA), the direct activator of protein kinase C (PKC), accelerated the endocytosis and recycling of the complex in a dose-dependent manner. W-7, the calmodulin antagonist, and GF109203X, a selected cell-permeant inhibitor of PKC, can reverse the acceleration. Analysis of actin polymerization in controlled, [Ca2+](i)-elevated and W-7-treated cells revealed that the actin polymerization is enhanced as [Ca2+](i) is raised, but reduced by W-7. The results suggest that the regulation of actin polymerization by intracellular Ca2+ may play a central role in Ca2+-dependent iron uptake.
URI: http://hdl.handle.net/10397/30164
ISSN: 0143-4160
DOI: 10.1016/S0143-4160(02)00240-3
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

13
Last Week
0
Last month
Citations as of Dec 6, 2017

WEB OF SCIENCETM
Citations

13
Last Week
0
Last month
0
Citations as of Dec 10, 2017

Page view(s)

44
Last Week
1
Last month
Checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.