Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30139
Title: Systematic sequence analysis of the human fucosyltransferase 2 (FUT2) gene identifies novel sequence variations and alleles
Authors: Yip, SP 
Lai, SK
Wong, ML
Issue Date: 2007
Publisher: Blackwell Publishing
Source: Transfusion, 2007, v. 47, no. 8, p. 1369-1380 How to cite?
Journal: Transfusion 
Abstract: BACKGROUND: The FUT2 gene regulates the expression of ABH antigens in body secretions and hence controls the secretor status. It is highly polymorphic. STUDY DESIGN AND METHODS: Healthy Chinese (n = 79) and Caucasian (n = 20) subjects were recruited for this study. Lewis blood group and secretor status were determined. The entire FUT2 coding region was amplified and screened for sequence variations by single-strand conformation polymorphism analysis under four different conditions to ensure comprehensive detection, and representative samples with distinct banding patterns were characterized by DNA sequencing. The haplotypes of novel alleles were determined by sequencing of cloned inserts. RESULTS: Seventeen single-nucleotide polymorphisms and one 3-base duplicating insertion were identified. Six novel FUT2 alleles each found in a heterozygous individual were defined by six novel sequence variations: 210A>G (T70T), 380G>A (R127H), 572G>A (R191Q), 748_750dupGTG (V250dup), 853G>A (A285T), and 855A>C (A285A). The three most common alleles were Se, Se 357, and Se357,385 in Chinese persons (total frequency, 94%) and se428, Se357, and Se in Caucasian persons (total frequency, 85%). The FUT2 genotypes correlated with Lewis phenotypes and secretor status. CONCLUSION: This study identified 18 sequence variations in the FUT2 gene, and 6 were novel. The frequencies of alleles and genotypes were also determined in Chinese and Caucasian persons.
URI: http://hdl.handle.net/10397/30139
DOI: 10.1111/j.1537-2995.2007.01280.x
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

16
Last Week
0
Last month
0
Citations as of May 22, 2017

WEB OF SCIENCETM
Citations

14
Last Week
0
Last month
0
Citations as of May 14, 2017

Page view(s)

43
Last Week
2
Last month
Checked on May 21, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.