Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/30006
Title: Exercise decreases cytosolic aconitase activity in the liver, spleen, and bone marrow in rats
Authors: Ho, KP
Xiao, DS
Ke, Y
Qian, ZM
Keywords: Cytosolic aconitase activity (CAA)
Iron metabolism
Iron-regulatory protein 1
L-NAME (N-nitro-L-arginine methylester)
Nitric oxide (NO)
Strenuous exercise
Issue Date: 2001
Publisher: Academic Press
Source: Biochemical and biophysical research communications, 2001, v. 282, no. 1, p. 264-267 How to cite?
Journal: Biochemical and biophysical research communications 
Abstract: Effects of strenuous exercise on cytosolic aconitase activity (CAA) were investigated in this study. Female Sprague-Dawley rats were randomly assigned to four groups: S1 (Sedentary), S2 (Sedentary+L-NAME [N-nitro-L-arginine methyl ester]), E1 (Exercise), and E2 (Exercise+L-NAME). Rats in the E1 and E2 groups swam for 2 h/day for 3 months. L-NAME (an inhibitor of NOS) in drinking water (1 mg/ml) was administered to rats in the S2 and E2 groups for the same period. At the end of the third month, the CAA in the liver, spleen, and bone marrow cells was measured. In the exercise group (E1), CAA in the liver, spleen, and bone marrow cells was 19.99±1.49, 1.61±0.13, and 0.59±0.09 mU/mg protein, respectively. These values were significantly lower than the corresponding sedentary values in the S1 group (33.96±1.38, 3.96±0.19, and 3.20±0.18 mU/mg protein) (P<0.01, 0.001, and 0.001, respectively). The treatment of L-NAME led to a significant increase in tissue CAA in the sedentary rats (S2). Also, the significantly higher CAA in the liver, spleen, and bone marrow cells was found in the exercised rats treated with L-NAME (E2) (29.50±1.27, 2.89±0.25, and 1.34±0.20 mU/mg) than without L-NAME (E1) (P<0.01, 0.01, 0.05, respectively). However, the values in the E2 group were still significantly lower than those in the S1 group (P<0.05, 0.01, and 0.001, respectively). This indicates that L-NAME treatment can partly recover the decreased CA in tissues in the exercised rats. These results provide evidence for the existence of the increased activity of IRP1 (iron regulatory protein 1) that is probably induced by the increased nitric oxide production in the strenuously exercised rats.
URI: http://hdl.handle.net/10397/30006
ISSN: 0006-291X
EISSN: 1090-2104
DOI: 10.1006/bbrc.2001.4536
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