Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29814
Title: A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants
Authors: Wan, H
Liu, D
Yu, X
Sun, H
Li, Y
Keywords: Antioxidants
Caco-2 cell-based antioxidant activity (CAA)
Caco-2 cells
Oxygen radical absorbance capacity (ORAC)
Quantitative assay
Issue Date: 2015
Publisher: Elsevier
Source: Food chemistry, 2015, v. 175, p. 601-608 How to cite?
Journal: Food chemistry 
Abstract: A Caco-2 cell-based antioxidant activity (CAA) assay for quantitative evaluation of antioxidants was developed by optimizing seeding density and culture time of Caco-2 cells, incubation time and concentration of fluorescent probe (2′,7′-dichlorofluorescin diacetate, DCFH-DA), incubation way and incubation time of antioxidants (pure phytochemicals) and DCFH-DA with cells, and detection time of fluorescence. Results showed that the CAA assay was of good reproducibility and could be used to evaluate the antioxidant activity of antioxidants at the following conditions: seeding density of 5 × 104/well, cell culture time of 24 h, co-incubation of 60 μM DCFH-DA and pure phytochemicals with Caco-2 cells for 20 min and fluorescence recorded for 90 min. Additionally, a significant correlation was observed between CAA values and rat plasma ORAC values following the intake of antioxidants for selected pure phytochemicals (R2 = 0.815, p < 0.01), demonstrating the good biological relevance of CAA assay.
URI: http://hdl.handle.net/10397/29814
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2014.11.128
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