Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29806
Title: Effects of Tween 80 and pH on mycelial pellets and exopolysaccharide production in liquid culture of a medicinal fungus
Authors: Liu, YS
Wu, JY 
Keywords: Cordyceps sinensis
Liquid culture
Exopolysaccharide
Tween 80
Mycelial morphology
Issue Date: 2012
Publisher: Springer
Source: Journal of industrial microbiology & biotechnology, 2012, v. 39, no. 4, p. 623-628 How to cite?
Journal: Journal of industrial microbiology & biotechnology 
Abstract: This study investigated the effects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS) production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l(-1) glucose and 6 g l(-1) peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter. The mycelial pellets became less uniform at pH (4.0-5.0) lower than the optimum (pH 6.0) or turned to filamentous form at higher pH (8-9). Surfactants added to the medium inhibited pellet formation, resulting in smaller and looser pellets. Tween 80 exhibited a remarkable promoting effect on EPS production, increasing the EPS yield more than twofold at 1.5% (w/v), which was most probably attributed to the stimulation of EPS biosynthesis and release from the fungal cells by Tween 80.
URI: http://hdl.handle.net/10397/29806
ISSN: 1367-5435
EISSN: 1476-5535
DOI: 10.1007/s10295-011-1066-9
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