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Title: Clonal expansions of cytotoxic T cells exist in the blood of patients with Waldenstrom macroglobulinemia but exhibit anergic properties and are eliminated by nucleoside analogue therapy
Authors: Li, J
Sze, DM
Brown, RD
Cowley, MJ
Kaplan, W
Mo, SL
Yang, S
Aklilu, E
Kabani, K
Loh, YS
Yamagishi, T
Chen, Y
Ho, PJ
Joshua, DE
Issue Date: 2010
Publisher: American Society of Hematology
Source: Blood, 2010, v. 115, no. 17, p. 3580-3588 How to cite?
Journal: Blood 
Abstract: T cells contribute to host-tumor interactions in patients with monoclonal gammopathies. Expansions of CD8(+)CD57(+) T-cell receptor V beta-positive (TCRV beta(+))-restricted cytotoxic T-cell (CTL) clones are found in 48% of patients with multiple myeloma and confer a favorable prognosis. We now report that CTL clones with varying TCRV beta repertoire are present in 70% of patients with Waldenstrom macroglobulinemia (WM; n = 20). Previous nucleoside analog (NA) therapy, associated with increased incidence of transformation to aggressive lymphoma, significantly influenced the presence of TCRV beta expansions (chi(2) = 11.6; P < .001), as 83% of patients without (n = 6) and only 7% with (n = 14) TCRV beta expansions had received NA. Clonality of CD3(+)CD8(+)CD57(+) TCRV beta(+) restricted CTLs was confirmed by TCRV beta CDR3 size analysis and direct sequencing. The differential expression of CD3(+)CD8(+)CD57(+) TCRV beta(+) cells was profiled using DNA microarrays and validated at mRNA and protein level. By gene set enrichment analysis, CTL clones expressed not only genes from cytotoxic pathways (GZMB, PRF1, FGFBP2) but also genes that suppress apoptosis, inhibit proliferation, arrest cell-cycle G1/S transition, and activate T cells (RAS, CSK, and TOB pathways). Proliferation tracking after stimulation confirmed their anergic state. Our studies demonstrate the incidence, NA sensitivity, and nature of clonal CTLs in WM and highlight mechanisms that cause anergy in these cells.
ISSN: 0006-4971
EISSN: 1528-0020
DOI: 10.1182/blood-2009-10-246991
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