Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29497
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dc.contributor.authorBenzie, IFFen_US
dc.contributor.authorStrain, JJen_US
dc.date.accessioned2014-12-19T07:06:24Z-
dc.date.available2014-12-19T07:06:24Z-
dc.date.issued1996-
dc.identifier.citationAnalytical biochemistry, 1996, v. 239, no. 1, p. 70-76en_US
dc.identifier.issn0003-2697-
dc.identifier.urihttp://hdl.handle.net/10397/29497-
dc.description.abstractA simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing 'antioxidant power.' Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, α-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 μmol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 μmol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.en_US
dc.description.sponsorshipDepartment of Health Technology and Informaticsen_US
dc.language.isoenen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.titleThe ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power' : the FRAP assayen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage70-
dc.identifier.epage76-
dc.identifier.volume239-
dc.identifier.issue1-
dc.identifier.doi10.1006/abio.1996.0292-
dc.identifier.isiWOS:A1996UY64800010-
dc.identifier.scopus2-s2.0-0030586361-
dc.identifier.pmid8660627-
item.fulltextFull Text (via PolyU elinks)-
crisitem.author.deptDepartment of Health Technology and Informatics-
crisitem.author.facultyFaculty of Health and Social Sciences-
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