Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29497
Title: The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power' : the FRAP assay
Authors: Benzie, IFF 
Strain, JJ
Issue Date: 1996
Source: Analytical biochemistry, 1996, v. 239, no. 1, p. 70-76 How to cite?
Journal: Analytical Biochemistry 
Abstract: A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing 'antioxidant power.' Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, α-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 μmol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 μmol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
URI: http://hdl.handle.net/10397/29497
ISSN: 0003-2697
DOI: 10.1006/abio.1996.0292
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