Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29478
Title: Conjugation of latent membrane protein (LMP)-2 epitope to gold nanoparticles as highly immunogenic multiple antigenic peptides for induction of Epstein-Barr virus-specific cytotoxic T-lymphocyte responses in vitro
Authors: Cheung, WH
Chan, VSF
Pang, HW
Wong, MK 
Guo, ZH
Tam, PKH
Che, CM
Lin, CL
Yu, WY 
Issue Date: 2009
Publisher: American Chemical Society
Source: Bioconjugate chemistry, 2009, v. 20, no. 1, p. 24-31 How to cite?
Journal: Bioconjugate chemistry 
Abstract: Nasopharyngeal carcinoma is a neoplasm with a high incidence in Southeast Asia, and it is strongly associated with Epstein-Barr virus (EBV) activation involving the expression of a weakly immunogenic protein, namely, latent membrane protein (LMP)-2. Previous immunological studies already identified the human leukocyte antigen (HLA)-All restricted peptide epitope (SSCSSCPLSK) in the LMP-2 antigen. In this work, we prepared gold nanoparticle (AuNP)-peptide conjugate 1 by treating the nanoparticles with the Af-cysteinated LMP-2 epitope. The AuNP-peptide conjugates have been characterized by TEM (15-24 nm in diameter) and UV-vis spectroscopy (surface plasmon resonance absorption band at ?fmax = 520 nm). In the presence of a CALNN capping peptide, the AuNP-peptide conjugates are stable in solution without aggregation at room temperature for at least 48 h. By ELIspot studies, AuNP-peptide conjugate 1 was found to elicit a significantly stronger INF-?^ response [number of spot forming cells (SPC) = 727 ?? 198
URI: http://hdl.handle.net/10397/29478
ISSN: 1043-1802
DOI: 10.1021/bc800167q
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