Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29148
Title: Expression and association of TRPC1 with TRPC3 during skeletal myogenesis
Authors: Cheung, KK
Yeung, SS 
Au, SW
Lam, LS
Dai, ZQ
Li, YH
Yeung, EW 
Keywords: Myogenesis
Skeletal muscle
Store-operated channel
TRPC1
TRPC3
Issue Date: 2011
Publisher: John Wiley & Sons
Source: Muscle and nerve, 2011, v. 44, no. 3, p. 358-365 How to cite?
Journal: Muscle and nerve 
Abstract: Introduction: TRPC1 and TRPC3 proteins are widely expressed in skeletal muscles in forming calcium-permeable channels. Herein we characterize the expression pattern of TRPC transcripts during skeletal myogenesis in C2C12 myo-blasts. Methods: We used polymerase chain reaction and Western blotting to detect expression levels, immunohistochemistry for subcellular localization, and co-immunoprecipitation techniques to assess interaction. Results: TRPC1 localizes to the cytoplasm and is enriched in the perinuclear region in undifferentiated myoblasts. Expression of TRPC1 increases significantly during myogenesis and resides mainly in differentiated myocytes and myotubes. TRPC3 is absent in undifferentiated myoblasts, is dramatically upregulated in differentiated culture, and is preferentially expressed in myotubes. Physical interaction of TRPC1-TRPC3 was observed, suggesting the possible existence of heteromers. Conclusions: Expression of TRPC1 and TRPC3 is tightly regulated during myogensis. Evidence of TRPC1-TRPC3 interaction was first demonstrated in a muscle cell line. The functional consequences of this interaction remain to be established.
URI: http://hdl.handle.net/10397/29148
ISSN: 0148-639X
EISSN: 1097-4598
DOI: 10.1002/mus.22060
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