Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/29059
Title: Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus
Authors: Huang, QL
Siu, KC
Wang, WQ
Cheung, YC
Wu, JY 
Keywords: Antioxidant activity
Cordyceps sinensis
Ethanol precipitation
Liquid fermentation
Polysaccharide-protein complex
Issue Date: 2013
Publisher: Elsevier
Source: Process biochemistry, 2013, v. 48, no. 2, p. 380-386 How to cite?
Journal: Process biochemistry 
Abstract: Exopolysaccharide (EPS) fractions P1/5, P2/5, P 1, P2 and P5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P1/5 and P2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [η] 2025 mL/g; hydrodynamic radius R h 905 nm). The fraction attained at a higher ethanol volume ratio had a much higher protein and lower carbohydrate content, and a smaller molecular size. In particular, P5 was composed of mainly protein with an average molecular weight 16 kDa, [η] 4.3 mL/g and Rh 23.5 nm. The antioxidant activities of EPS fractions showed a significant dependence on the protein content, being negligible of P1/5 and P2/5, low to moderate of P1 and P2, and very strong of P5. Gradient ethanol precipitation was proven a simple and workable method for initial fractionation of polysaccharides, proteins and their complexes with different molecular sizes and for further identification of the bioactive components.
URI: http://hdl.handle.net/10397/29059
ISSN: 1359-5113
EISSN: 1873-3298
DOI: 10.1016/j.procbio.2013.01.001
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