Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28756
Title: Hyperthermic preconditioning protects astrocytes from ischemia/reperfusion injury by up-regulation of HIF-1 alpha expression and binding activity
Authors: Du, F
Zhu, L
Qian, ZM
Wu, XM
Yung, WH
Ke, Y
Keywords: Apoptosis
Cell viability
Hyperthermiac preconditioning
Hypoxia-inducible factor-1 alpha (HIF-1 alpha)
Ischemia/reperfusion
Lactate dehydrogenase (LDH)
Reactive astrocytes
Issue Date: 2010
Publisher: Elsevier
Source: Biochimica et biophysica acta. Molecular basis of disease, 2010, v. 1802, no. 11, p. 1048-1053 How to cite?
Journal: Biochimica et biophysica acta. Molecular basis of disease 
Abstract: It has been demonstrated that hypoxia-inducible factor-1 alpha (HIF-1 alpha) mediates ischemic tolerance induced by hypoxia/ischemia or pharmacological preconditioning. In addition, preconditioning stimuli can be cross-tolerant, safeguarding against other types of injury. We therefore hypothesized HIF-1 alpha might also be associated with ischemic tolerance induced by hyperthermic preconditioning. In the present study, we demonstrated for the first time that 6. h of hyperthermia (38 °C or 40 °C) could induce a characteristic "reactive" morphology and a significant increase in the expression of bystin in astrocytes. We also showed that pre-treatment with 6. h of hyperthermia resulted in a significant increase in cell viability and a remarkable decrease in lactate dehydrogenase (LDH) release and apoptosis development in the astrocytes that were exposed to 24. h of ischemia and a subsequent 24. h of reperfusion. Analysis of mechanisms showed that hyperthermia could lead to a significant increase in HIF-1 alpha expression and also the HIF-1 binding activity in the ischemia/reperfusion astrocytes. The data provide evidence to our hypothesis that the up-regulation of HIF-1 alpha is associated with the protective effects of hyperthermic preconditioning on astrocytes against ischemia/reperfusion injury.
URI: http://hdl.handle.net/10397/28756
ISSN: 0925-4439
DOI: 10.1016/j.bbadis.2010.06.013
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