Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28576
Title: Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding
Authors: Wang, C
Wu, J 
Mei, X
Issue Date: 2001
Publisher: Amer Chemical Soc
Source: Biotechnology progress, 2001, v. 17, no. 1, p. 89-94 How to cite?
Journal: Biotechnology Progress 
Abstract: Suspension culture of Taxus chinensis cells was carried out in aqueous-organic two-phase systems for the production and in situ solvent extraction of taxol (paclitaxel). Three organic solvents, hexadecane, decanol, and dibutylphthalate, were tested at 5-20% (v/v) in the culture liquid. All of these solvents stimulated taxol release and the yield per cell, though decanol and higher concentrations of the other two solvents depressed biomass growth significantly. Ten percent dibutylphthalate was the optimal solvent for improving taxol production and release with minimal cell growth inhibition. The time of solvent addition to the culture also affected taxol production, with the addition during the late-log growth phase being most favorable. By feeding sucrose to the culture near the stationary growth phase, the cell growth and taxol production period was extended from 27 to 42 days. The combining of the two-phase culture and sucrose feeding increased the taxol yield by about 6-fold compared with the single-phase batch culture, to 36.0 ± 3.5 mg/L, with up to 63% taxol released. This study shows that in situ solvent extraction combined with nutrient feeding is an effective process strategy for production and recovery of secondary metabolites in plant cell suspension culture.
URI: http://hdl.handle.net/10397/28576
ISSN: 8756-7938
DOI: 10.1021/bp0001359
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

57
Last Week
0
Last month
0
Citations as of Aug 12, 2017

WEB OF SCIENCETM
Citations

53
Last Week
0
Last month
1
Citations as of Aug 12, 2017

Page view(s)

30
Last Week
0
Last month
Checked on Aug 14, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.