Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28531
Title: Effects of dietary antioxidants on human DNA ex vivo
Authors: Szeto, YT
Benzie, IFF 
Keywords: Antioxidant
Caffeic acid
Comet assay
DNA
Oxidative stress
Quercetin
Issue Date: 2002
Source: Free radical research, 2002, v. 36, no. 1, p. 113-118 How to cite?
Journal: Free Radical Research 
Abstract: The protective effect of fruits and vegetables against cancer is well established. It is believed that this effect is mediated by antioxidants and decreased oxidative damage to DNA. However, the identity of the antioxidant(s) responsible is not clear. Moreover, a potentially damaging pro-oxidant effect of some antioxidants has been reported. In this study the ex vivo effects of several dietary antioxidants, including quercetin, various catechins, ascorbic acid and α-tocopherol, were investigated, at concentrations up to 200 μM, using the single cell gel electrophoresis (comet) assay for DNA damage. Lymphocytes from three healthy subjects were pre-incubated with these antioxidants, and the comet assay was performed on treated, untreated, challenged and unchallenged cells in parallel, oxidant challenge being induced by 5 min exposure to hydrogen peroxide (final concentrations H2O2: 30, 45, or 60 μM). Results using this ex vivo cellular assay showed protection by some antioxidants (quercetin, caffeic acid), no effect by some (catechin, epicatechin, catechin gallate, epicatechin gallate) and an apparently damaging effect by others (epigallocatechin, epigallocatechin gallate). Damage may have been caused by production of H2O2 from these polyphenolics. Neither ascorbic acid nor α-tocopherol protected or damaged DNA. Further study of the role of quercetin and caffeic acid in DNA protection is needed.
URI: http://hdl.handle.net/10397/28531
ISSN: 1071-5762
DOI: 10.1080/10715760210161
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