Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28510
Title: Ultra-stable oligonucleotide-gold and -silver nanoparticle conjugates prepared by a facile silica reinforcement method
Authors: Wong, JF
Yip, S 
Lee, TH 
Keywords: Oligonucleotide-nanoparticle conjugate
Silica reinforcement coating
Gold nanoparticle
Silver nanoparticle
Issue Date: 2012
Source: Nano research, 2012, v. 5, no. 9, p. 585-594 How to cite?
Journal: Nano research 
Abstract: We report a simple method of enhancing the chemical stability of monothiol-modified oligonucleotide-gold and -silver nanoparticle conjugates by a thin silica reinforcement coating. Conventional conjugates prepared by chemisorption of monothiol-modified oligonucleotides onto nanoparticle surfaces undergo rapid aggregation in the presence of thiol-containing small molecules (e.g., dithiothreitol) due to ligand exchange reactions. When the conjugates are treated with (3-mercaptopropyl)trimethoxysilane, a thin silica layer is formed on the nanoparticle surface, thereby entrapping and reinforcing the thiol-gold/-silver linkage. These silica-modified oligonucleotide-gold and -silver nanoparticle conjugates become much more stable toward dithiothreitol as compared to the unmodified conjugates. Moreover, the silica layer significantly hinders the gold/silver core from oxidative dissolution by sodium cyanide. Importantly, the unique hybridization-induced color change property of the oligonucleotide-gold and -silver nanoparticle conjugates is preserved even under harsh condition (i.e., high concentrations of dithiothreitol). Taken together, these ultra-stable oligonucleotide-nanoparticle conjugates hold promise for new diagnostics and therapeutics.
URI: http://hdl.handle.net/10397/28510
ISSN: 1998-0124
DOI: 10.1007/s12274-012-0244-z
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