Please use this identifier to cite or link to this item:
Title: Ultra-stable oligonucleotide-gold and -silver nanoparticle conjugates prepared by a facile silica reinforcement method
Authors: Wong, JF
Yip, S 
Lee, TH 
Keywords: Oligonucleotide-nanoparticle conjugate
Silica reinforcement coating
Gold nanoparticle
Silver nanoparticle
Issue Date: 2012
Source: Nano research, 2012, v. 5, no. 9, p. 585-594 How to cite?
Journal: Nano research 
Abstract: We report a simple method of enhancing the chemical stability of monothiol-modified oligonucleotide-gold and -silver nanoparticle conjugates by a thin silica reinforcement coating. Conventional conjugates prepared by chemisorption of monothiol-modified oligonucleotides onto nanoparticle surfaces undergo rapid aggregation in the presence of thiol-containing small molecules (e.g., dithiothreitol) due to ligand exchange reactions. When the conjugates are treated with (3-mercaptopropyl)trimethoxysilane, a thin silica layer is formed on the nanoparticle surface, thereby entrapping and reinforcing the thiol-gold/-silver linkage. These silica-modified oligonucleotide-gold and -silver nanoparticle conjugates become much more stable toward dithiothreitol as compared to the unmodified conjugates. Moreover, the silica layer significantly hinders the gold/silver core from oxidative dissolution by sodium cyanide. Importantly, the unique hybridization-induced color change property of the oligonucleotide-gold and -silver nanoparticle conjugates is preserved even under harsh condition (i.e., high concentrations of dithiothreitol). Taken together, these ultra-stable oligonucleotide-nanoparticle conjugates hold promise for new diagnostics and therapeutics.
ISSN: 1998-0124
DOI: 10.1007/s12274-012-0244-z
Appears in Collections:Journal/Magazine Article

View full-text via PolyU eLinks SFX Query
Show full item record


Citations as of Mar 23, 2017

Page view(s)

Last Week
Last month
Checked on Mar 19, 2017

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.