Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28350
Title: Enhancement of saponin production in Panax ginseng cell culture by osmotic stress and nutrient feeding
Authors: Wu, JY 
Wong, K
Ho, KP
Zhou, LG
Keywords: Cell culture
Nutrient feeding
Osmotic stress
Panax ginseng
Saponin
Issue Date: 2005
Publisher: Elsevier
Source: Enzyme and microbial technology, 2005, v. 36, no. 1, p. 133-138 How to cite?
Journal: Enzyme and microbial technology 
Abstract: Osmotic stress at elevated osmotic pressure was used to stimulate saponin production in Panax ginseng cell cultures. The addition of 0.3 M sorbitol to culture at the time of inoculation, raising the medium osmolality by about 75% (from 150 to 260 mmol/kg), enhanced the saponin accumulation but depressed the cell growth, resulting in no improvement of the volumetric saponin yield of culture. The feeding of sorbitol (0.2-0.3 M, 36.4-54.6 g/L) to culture during the stationary growth phase increased both the saponin content of cell (45-50%) and the volumetric yield (36-38%). With combined feeding of sorbitol (0.2 M) and growth nutrients, sucrose (30 g/L) + casein hydrolysate (0.5 g/L), about 2 days before the stationary phase, the volumetric saponin yield was increased 3.5-fold, 1130.8 mg/L versus the control culture of 251.2 mg/L. Sorbitol in the culture also induced two characteristic stress responses of plant cells, the increase in phenylalanine ammonium-lyase (PAL) activity and the production of reactive oxygen species. This suggests that the stimulated saponin accumulation was part of the cell response to the osmotic stress.
URI: http://hdl.handle.net/10397/28350
ISSN: 0141-0229
DOI: 10.1016/j.enzmictec.2004.07.010
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