Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28034
Title: Cloning and expression of the PHA synthase genes phaC1 and phaC1AB into Bacillus subtilis
Authors: Wang, Y
Ruan, L
Chua, H
Yu, PHF
Keywords: Bacillus subtilis
Cloning and expression
P(HB-co-mclHA)
PHA synthase gene
Polyhydroxyalkanoates (PHAs)
Issue Date: 2006
Source: World journal of microbiology and biotechnology, 2006, v. 22, no. 6, p. 559-563 How to cite?
Journal: World Journal of Microbiology and Biotechnology 
Abstract: Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1 and pBE2C1AB. The two plasmids were inserted into Bacillus subtilis DB104 and generated Bacillus subtilis/pBE2C1 and Bacillus subtilis/pBE2C1AB. The two recombinant strains were subjected to fermentation and showed PHA accumulation, the first reported example of medium-chain-length-PHA production in Bacillus subtilis. GC analysis identified the compound produced by Bacillus subtilis/pBE2C1 was a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer while that produced by Bacillus subtilis/pBE2C1AB was a hydroxybutyrate-co-hydroxydecanoate-co- hydroxydodecanoate (HB-HD-HDD) polymer. The results also showed that the recombinant B. subtilis could utilize the malt waste in the medium as a carbon source better than that of glucose and thus could substantially lower the cost of production of PHA.
URI: http://hdl.handle.net/10397/28034
ISSN: 0959-3993
DOI: 10.1007/s11274-005-9071-7
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