Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27938
Title: A PDMS microfluidic impedance immunosensor for E. coli O157:H7 and Staphylococcus aureus detection via antibody-immobilized nanoporous membrane
Authors: Tan, F
Leung, PHM 
Liu, ZB
Zhang, Y
Xiao, L
Ye, W
Zhang, X
Li, Y 
Yang, M 
Keywords: Microfluidic immunosensor
Nanoporous membrane
Electrochemical impedance spectrum
Antibody immobilization
Bacteria detection
Issue Date: 2011
Publisher: Elsevier
Source: Sensors and actuators. B, Chemical, 2011, v. 159, no. 1, p. 328-335 How to cite?
Journal: Sensors and actuators. B, Chemical 
Abstract: In this article, a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane was developed for rapid detection of foodborne pathogens Escherichia coli O157:H7 and Staphylococcus aureus with electrochemical impedance spectrum. Firstly, antibodies to the targeted bacteria were covalently immobilized on the nanoporous alumina membranes via self assembled (3-glycidoxypropyl)trimethoxysilane (GPMS) silane. Then, the impedance spectrum was recorded for bacteria detection ranging from 1 Hz to 100 kHz. The maximum impedance amplitude change for these two food pathogens was around 100 Hz. This microfluidic immunosensor based on nanoporous membrane impedance spectrum could achieve rapid bacteria detection within 2 h with a high sensitivity of 102 CFU/ml. Cross-bacteria experiments for E. coli O157:H7 and S. aureus were also explored to testify the specificity. The results showed that impedance amplitude at 100 Hz had a significant reduction in binding of bacteria when the membrane was exposed to non-specific bacteria.
URI: http://hdl.handle.net/10397/27938
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2011.06.074
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