Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27823
Title: A nanoporous membrane based impedance sensing platform for DNA sensing with gold nanoparticle amplification
Authors: Ye, WW
Shi, JY
Chan, CY
Zhang, Y
Yang, M 
Keywords: DNA sensor
Gold nanoparticles
Nanoporous alumina membrane
Silver enhancement
Issue Date: 2013
Publisher: Elsevier
Journal: Sensors and actuators. B, Chemical 
Abstract: Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane based impedance sensing platform with gold nanoparticle tags amplification was developed for DNA sensing. Single stranded DNA (ssDNA) probes were first covalently immobilized in nanopore walls via chemical linkers. DNA hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal could be further amplified by gold nanoparticles (AuNPs) conjugation and silver catalytic deposition method to reach a limit of detection (LOD) of 50 pM. This platform is promising for simple, rapid and sensitive DNA detection.
URI: http://hdl.handle.net/10397/27823
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2013.09.115
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