Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27708
Title: Mitogen-activated protein kinase (MAPK) pathway mediates the oestrogen-like activities of ginsenoside Rg1 in human breast cancer (MCF-7) cells
Authors: Lau, WS
Chen, WF
Chan, RYK
Guo, DA
Wong, MS 
Keywords: ER£\
Ginsenoside Rg1
MAPK
Phytoestrogens
Issue Date: 2009
Publisher: Wiley-Blackwell
Source: British journal of pharmacology, 2009, v. 156, no. 7, p. 1136-1146 How to cite?
Journal: British journal of pharmacology 
Abstract: Background and purpose: The present study was designed to determine how ginsenoside Rg1, an active ingredient in ginseng root, exerts its oestrogenic effects. We hypothesize that Rg1 may exert oestrogen-like actions in MCF-7 cells by activating the mitogen-activated protein kinase (MAPK) pathway in a ligand-independent manner. Experimental approach: MCF-7 cells were co-incubated with the MAPK inhibitor PD98059 to determine whether the stimulant effects of Rg1 on cell proliferation, the induction of IGF-IR and pS2, the functional transactivation of oestrogen receptor-£\ (ER£\), as well as ER£\ phosphorylation are dependent on MAPK. The time-dependent responses of mitogen-activated protein kinase kinase (MEK) and extracellular signal-regulated protein kinase (ERK) to Rg1 in MCF-7 cells were studied. The responses of MEK phosphorylation to Rg1 in oestrogen receptor (ER)-negative HEK293 cells were also determined. The effects of Rg1 on cell proliferation and IGF-IR protein expression were studied in the presence of tyrosine kinase inhibitor genistein to elucidate the involvement of tyrosine kinase in mediating these effects. Key results: The oestrogenic effects of Rg1 in MCF-7 cells were abolished in the presence of PD98059. Rg1 could induce MEK protein expression and the phosphorylation level of MEK and ERK significantly in a time- and dose-dependent manner. Rg1
URI: http://hdl.handle.net/10397/27708
ISSN: 0007-1188
EISSN: 1476-5381
DOI: 10.1111/j.1476-5381.2009.00123.x
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