Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27597
Title: Involvement of nitric oxide in elicitor-induced defense responses and secondary metabolism of Taxus chinensis cells
Authors: Wang, JW
Wu, JY 
Keywords: Fungal elicitor
K +/H + fluxes
Nitric oxide
Oxidative burst
Paclitaxel
Taxus cells
Issue Date: 2004
Publisher: Academic Press
Source: Nitric oxide : biology and chemistry, 2004, v. 11, no. 4, p. 298-306 How to cite?
Journal: Nitric oxide : biology and chemistry 
Abstract: This work was to characterize the generation of nitric oxide (NO) in Taxus chinensis cells induced by a fungal elicitor extracted from Fusarium oxysporum mycelium and the signal role of NO in the elicitation of plant defense responses and secondary metabolite accumulation. The fungal elicitor at 10-100 μg/ml (carbohydrate equivalent) induced a rapid and dose-dependent NO production in the Taxus cell culture, which exhibited a biphasic time course, reaching the first plateau within 1 h and the second within 12 h of elicitor treatment. The NO donor sodium nitroprusside potentiated elicitor-induced H 2O 2 production and cell death but had little influence on elicitor-induced membrane K + efflux and H + influx (medium alkalinization). NO inhibitors N ω-nitro-l-arginine and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide partially blocked the elicitor-induced H 2O 2 production and membrane ion fluxes. Moreover, the NO inhibitors suppressed elicitor-induced activation of phenylalanine ammonium-lyase and accumulation of diterpenoid taxanes (paclitaxel and baccatin III). These results suggest that NO plays a signal role in the elicitor-induced responses and secondary metabolism activities in the Taxus cells.
URI: http://hdl.handle.net/10397/27597
ISSN: 1089-8603
DOI: 10.1016/j.niox.2004.10.003
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