Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27223
Title: Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response
Authors: Waugh, DG
Lawrence, J
Shukla, P
Chan, C
Hussain, I
Man, HC 
Smith, GC
Keywords: Cell viability
CO2 laser
Fiber laser
Laser surface treatment
MTT
Stem cells
Issue Date: 2015
Publisher: SPIE-International Society for Optical Engineering
Source: Proceedings of SPIE : the International Society for Optical Engineering, 2015, v. 9657, 96570L How to cite?
Journal: Proceedings of SPIE : the International Society for Optical Engineering 
Abstract: Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.
Description: 4th Industrial Laser Applications Symposium, ILAS 2015, 17-18 March 2015
URI: http://hdl.handle.net/10397/27223
ISBN: 9781630000000
ISSN: 0277-786X
EISSN: 1996-756X
DOI: 10.1117/12.2182432
Appears in Collections:Conference Paper

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