Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27022
Title: Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor
Authors: Chan, KY
Ye, WW
Zhang, Y
Xiao, LD
Leung, PHM 
Li, Y 
Yang, M 
Keywords: Magnetic bead concentration
Bacteria detection
Nanoporous membrane
Impedance sensor
Issue Date: 2013
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2013, v. 41, p. 532-537 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: In this paper, biofunctional magnetic beads were investigated for bacterial cells concentration in a nanoporous alumina membrane based immunosensor for ultra-sensitive detection of E. coli O157:H7. The specific antibody modified magnetic beads were used for concentration of E coli O157:H7 from samples in a small region to enhance sensitivity. The magnetic bead conjugated E. coli O157:H7 cells were then captured on the nanoporous alumina membrane with immobilized antibody via assembled PEG-silane linker. Scanning electron microscopy and fluorescent microscopy were used to demonstrate the magnetic bead-bacteria cell conjugation and bacteria cells magnetic concentration, respectively. Impedance spectroscopy was used to monitor the pure E coli O157:H7 cells and magnetic bead conjugated E coli O157:H7 cells binding on antibody immobilized nanoporous membrane with or without magnetic field. Compared with direct detection of pure bacteria cells, this method via magnetic bead conjugation and concentration demonstrated the ultrasensitivity of 10 CFU/mL for E coli O157:H7 detection.
URI: http://hdl.handle.net/10397/27022
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/j.bios.2012.09.016
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

35
Last Week
0
Last month
Citations as of Apr 30, 2016

WEB OF SCIENCETM
Citations

46
Last Week
0
Last month
2
Citations as of Aug 14, 2017

Page view(s)

54
Last Week
3
Last month
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.