Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/27017
Title: Role of histone deacetylase inhibitors in the aging of human umbilical cord mesenchymal stem cells
Authors: Wang, Y
Chen, T
Yan, H
Qi, H
Deng, C
Ye, T 
Zhou, S
Li, FR
Keywords: Epigenetic modification
Histone deacetylase inhibitor
Human umbilical cord-mesenchymal stem cell
Proliferation
Issue Date: 2013
Source: Journal of cellular biochemistry, 2013, v. 114, no. 10, p. 2231-2239 How to cite?
Journal: Journal of Cellular Biochemistry 
Abstract: Mesenchymal stem cells (MSCs) are self-renewing cells that exhibit differentiation capacity and immune regulation ability. These versatile cells have a wide range of potential applications. However, the spontaneous differentiation and aging of MSCs during long-term culturing restrict the amount of cells available for therapies and tissue engineering. Thus, maintaining the biological characteristics of MSCs during long-term culturing is crucial. Chromatic modification via epigenetic regulatory mechanisms (e.g., histone acetylation, deacetylation, and methylation) is crucial in stem cell pluripotency. We investigated the effects of largazole or trichostatin A (TSA), a novel histone deacetylase inhibitor (HDACi), against human umbilical cord (hUC)-MSCs aging. Results show that low concentrations of largazole or TSA can significantly improve hUC-MSCs proliferation and delay hUC-MSCs aging. Largazole can better improve MSCs proliferation than TSA. HDAC is modulate histone H3 acetylation and methylation in the telomerase reverse-transcriptase, octamer-binding transcription factor 4, Nanog, C-X-C chemokine receptor 4, alkaline phosphatase, and osteopontin genes. HDACis can promote hUC-MSCs proliferation and suppress hUC-MSCs spontaneous osteogenic differentiation. HDACis can affect histone H3 lysine 9 or 14 acetylation and histone H3 lysine 4 dimethylation, thus increasing the mRNA expression of pluripotent and proliferative genes and suppressing the spontaneous differentiation of hUC-MSCs. J. Cell. Biochem. 114: 2231-2239, 2013.
URI: http://hdl.handle.net/10397/27017
ISSN: 0730-2312
DOI: 10.1002/jcb.24569
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