Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/26964
Title: A switch-on fluorescence assay for bacterial β-lactamases with amyloid fibrils as fluorescence enhancer and visual tool
Authors: Zou, L
Cheong, WL
Chung, WH
Leung, YC 
Wong, KY 
Wong, MK 
Chan, PH 
Keywords: Amyloid fibrils
Antibiotics
Bacteria
Fluorescence
Sensors
Issue Date: 2010
Publisher: Wiley-VCH
Source: Chemistry - a European journal, 2010, v. 16, no. 45, p. 13367-13371 How to cite?
Journal: Chemistry - a European journal 
Abstract: Herein is described the development of a novel switch-on fluorescence assay for detecting β-lactamases. The fluorescence assay comprises two components: solid beads coated with a β-lactam antibiotic, which is linked to an environment-sensitive fluorophore (dansylaminothiophenol, DTA), and amyloid fibrils of hen lysozyme (acting as fluorescence enhancer and visual tool). In the presence of the clinically significant TEM-1 β-lactamase, the DTA-antibiotic complex on the solid beads is hydrolyzed, thus releasing the DTA dye into solution. The DTA dye is only weakly fluorescent in solution but gives strong green fluorescence upon binding to lysozyme fibrils. These strongly fluorescent DTA-bound fibrils can be easily visualized by the naked eye upon illumination of the sample with a simple UV lamp. The fluorescence assay can detect TEM-1 at low concentration (0.01a nM). In contrast, no observable fluorescence appears when the fluorescence assay is performed on samples without the TEM-1 β-lactamase. Visualizing bacterial β-lactamases: A switch-on fluorescence assay is developed for detecting bacterial β-lactamases. This simple fluorescence assay allows samples with β-lactamases to be conveniently identified through visual observation using amyloid fibrils as a fluorescence enhancer and visual tool.
URI: http://hdl.handle.net/10397/26964
ISSN: 0947-6539
EISSN: 1521-3765
DOI: 10.1002/chem.201001633
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

4
Last Week
0
Last month
0
Citations as of Aug 20, 2017

WEB OF SCIENCETM
Citations

3
Last Week
0
Last month
0
Citations as of Aug 20, 2017

Page view(s)

39
Last Week
1
Last month
Checked on Aug 21, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.